JUSTIN BIEBER's aides have denied reports the pop star spat Gatorade into a hotel gym user's water bottle.

Radio host Colette Harrington claims she was working out at the Ritz-Carlton Hotel gym in Charlotte, North Carolina on Tuesday (22Jan13) when Bieber entered with a trainer and a dancer.

Harrington plucked up courage to ask the pop star for an autograph but he refused. She then claims the Baby singer and his pals mocked her throughout her workout and one of the trio spat Gatorade into her water when she wasn't looking.

The radio presenter tells TMZ.com, "I always count out loud my reps. Then Justin starts imitating me and whenever I'd get to 20 he and his buddy would say, 'Shut the f**k up'."

Harrington filed a complaint with the hotel after the alleged incident, but bosses there refused to confirm or deny her story.

And Bieber's representative tells the website that the accuser has her facts all wrong, stating that the singer and his trainer were in the gym, and they were making snide comments - but not addressed at her or her work-out routine.